B.A., Agriculture, South West Forestry University, China
M.S., Agriculture, South West Forestry University, China
Ph.D., Plant Pathology, Kansas State University
The research of the Yang lab mainly focuses on 1) gaining a molecular understanding of host disease susceptibility and resistance to pathogenic microbes by using bacterial blight of rice as a disease model, and 2) developing and applying genome editing technologies for gene/trait discovery and for engineering improved traits such as disease resistance in crop plants.
Projects involve using rice as a workhorse to develop genome editing technologies and demonstrate their promising potential as biotech tools for basic and applied research in rice and other crop plants. With the increasingly available sequences of many eukaryotic genomes, technologies for genome editing have become the most promising and popular tools for scientists to understand such complex genomes and precisely and quickly manipulate them for good. Genome editing technologies allow precise, targeted genomic changes. TALENs and CRISPR/Cas represent the state-of-the-art genome editing technologies and hold immense promise for defining the genetic and molecular basis for valuable traits, as well as for the introgression of valuable genes/traits into cultivated varieties in a timely and economically sound manner.
By taking advantage of the genetic amenability of rice and its bacterial pathogen (Xanthomonas oryzae pv. oryzae, Xoo), the Yang lab, in collaboration with other groups, has made several breakthroughs in identifying key components (or virulence factors) that pathogenic Xoo strains use to hijack the host biological processes to condition a state of susceptibility in rice, and in identifying the rice counteracting strategies for disease resistance. The bacterial blight of rice represents one of the most well-studied crop diseases and is also well-known as a model for studying host/microbe interaction. TALEs (transcription activator-like effectors), as a group of pathogenesis factors and once translocated into the host cells from pathogens, recognize and activate host genes to condition disease susceptibility and also trigger host resistance responses dependent on the nature of target genes in plants. TALEs and their target genes have become the foci of the molecular battles between Xoo and rice. The continuing battles have led to incredibly diverse virulence mechanisms in pathogen and counteracting defense mechanisms in rice. Extensive effort in the Yang lab has been made to understand the TALE biology, identify host target genes, and elucidate their interaction and resulting physiological relevance to rice blight.