Education

Research Area

Proteomics using advanced mass spectrometry and 2D gel electrophoresis.

Research Description

I am particularly interested in protocol and techonology development for proteomics and mass spectrometry. We use mass spectrometry for protein identification, protein/peptide quantitation, and mapping sites of post-translational modifications and protein-protein interactions.

Metastable Crystallins: Structure and stabilization (NEI-NIH award R01EY023219)

Cataract and a host of other diseases result from abnormal interaction of proteins in the cells. The goals of this research are to understand lens protein aggregation in cataract formation and to develop and test lens protein alpha-crystallin-derived peptide as active mini-chaperones capable of suppressing protein aggregation. Understanding the structural changes in mutant proteins associated with cataract can help us learn why certain mutations in lens crystallins lead to cataract. Restoring the lost function in mutant crystallins may control the lens protein aggregation that characterizes cataract formation. Previous studies have shown that peptide chaperones stabilize unfolded proteins. This project will test the ability of peptide chaperones to stabilize and restore activity in mutant and modified lens crystallins. (9/1/2018 to 4/30/2022 – PI/PL: Sharma KK; Co-I: Mooney BP)

Selected Publications

Li F, Glinskii OV, Mooney BP, Rittenhouse-Olson K, Pienta KJ, Glinsky VV. (2017). Cell surface Thomsen-Friedenreich proteome profiling of metastatic prostate cancer cells reveals potential link with cancer stem cell-like phenotype. Oncotarget. 8(58):98598-98608. doi: 10.18632/oncotarget.21985. [PubMed]

Gruzdev V, Korkin D, Mooney BP, Havelund JF, Møller IM, Thelen JJ. (2017). Controlled modification of biomolecules by ultrashort laser pulses in polar liquids. Sci Rep. 7(1):5550. doi: 10.1038/s41598-017-05761-8. [PubMed]

Dahal D, Newton KJ, Mooney BP. (2016). Quantitative Proteomics of Zea mays Hybrids Exhibiting Different Levels of Heterosis. J Proteome Res. 15(8):2445-54. doi: 10.1021/acs.jproteome.5b01120. [PubMed]

Taylor I, Wang Y, Seitz K, Baer J, Bennewitz S, Mooney BP, Walker JC. (2016). Analysis of Phosphorylation of the Receptor-Like Protein Kinase HAESA during Arabidopsis Floral Abscission. PLoS One. 11(1):e0147203. doi: 10.1371/journal.pone.0147203. [PubMed]

Raju M, Mooney BP, Thakkar KM, Giblin FJ, Schey KL, Sharma KK. (2015). Role of αA-crystallin-derived αA66-80 peptide in guinea pig lens crystallin aggregation and insolubilization. Exp Eye Res. 132:151-60. doi: 10.1016/j.exer.2015.01.024. [PubMed]

Kannan R, Santhoshkumar P, Mooney BP, Sharma KK. (2013). Identification of subunit-subunit interaction sites in αA-WT crystallin and mutant αA-G98R crystallin using isotope-labeled cross-linker and mass spectrometry. PLoS One. 8(6):e65610. doi: 10.1371/journal.pone.0065610. [PubMed]

Kannan R, Santhoshkumar P, Mooney BP, Sharma KK. (2013). The αA66-80 peptide interacts with soluble α-crystallin and induces its aggregation and precipitation: a contribution to age-related cataract formation. Biochemistry. 52(21):3638-50. doi: 10.1021/bi301662w. [PubMed]